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mouse ccl17 tarc quantikine enzyme linked immunosorbent assay kit  (R&D Systems)


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    R&D Systems mouse ccl17 tarc quantikine enzyme linked immunosorbent assay kit
    Mouse Ccl17 Tarc Quantikine Enzyme Linked Immunosorbent Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl17+elisa+kits/Mouse+CCL17%2FTARC+Quantikine+ELISA+Kit/pm41957556-153-7-15
    Average 93 stars, based on 20 article reviews
    mouse ccl17 tarc quantikine enzyme linked immunosorbent assay kit - by Bioz Stars, 2026-09
    93/100 stars

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    Inhibitory effect of HCIE on AD-related cytokines expression in HaCaT cells. Gene expression of AD-related cytokines was measured using RT-PCR. The cells were pre-treated with the indicated concentrations of HCIE for 30 min and stimulated with TNF-α/IFN-γ (each 10 ng/mL). The relative mRNA levels of (A) IL-4, (B) IL-13, (C) MDC, (D) RANTES, (E) <t>TARC,</t> and (F) CXCL10 were measured, and mRNA was normalized to GAPDH. Secreted (G) RANTES and (H) TARC were evaluated using <t>ELISA.</t> (I) The percentage of relative gene expression was calculated. Data represented as mean ± SD ( n = 3) and the p values are presented on the graph as follows: ### p < 0.001 vs control group; ** p < 0.01, and *** p < 0.001 vs TNF-α/IFN-γ induced group.
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    Inhibitory effect of HCIE on AD-related cytokines expression in HaCaT cells. Gene expression of AD-related cytokines was measured using RT-PCR. The cells were pre-treated with the indicated concentrations of HCIE for 30 min and stimulated with TNF-α/IFN-γ (each 10 ng/mL). The relative mRNA levels of (A) IL-4, (B) IL-13, (C) MDC, (D) RANTES, (E) TARC, and (F) CXCL10 were measured, and mRNA was normalized to GAPDH. Secreted (G) RANTES and (H) TARC were evaluated using ELISA. (I) The percentage of relative gene expression was calculated. Data represented as mean ± SD ( n = 3) and the p values are presented on the graph as follows: ### p < 0.001 vs control group; ** p < 0.01, and *** p < 0.001 vs TNF-α/IFN-γ induced group.

    Journal: Frontiers in Pharmacology

    Article Title: Hybrid Cannabis sativa L. inflorescences exert an anti-inflammatory effect through the modulation of MAPK/NF-κB/NLRP3 inflammasome and JAK1/STAT6 pathway in HaCaT cells

    doi: 10.3389/fphar.2025.1617180

    Figure Lengend Snippet: Inhibitory effect of HCIE on AD-related cytokines expression in HaCaT cells. Gene expression of AD-related cytokines was measured using RT-PCR. The cells were pre-treated with the indicated concentrations of HCIE for 30 min and stimulated with TNF-α/IFN-γ (each 10 ng/mL). The relative mRNA levels of (A) IL-4, (B) IL-13, (C) MDC, (D) RANTES, (E) TARC, and (F) CXCL10 were measured, and mRNA was normalized to GAPDH. Secreted (G) RANTES and (H) TARC were evaluated using ELISA. (I) The percentage of relative gene expression was calculated. Data represented as mean ± SD ( n = 3) and the p values are presented on the graph as follows: ### p < 0.001 vs control group; ** p < 0.01, and *** p < 0.001 vs TNF-α/IFN-γ induced group.

    Article Snippet: For the measurement of secreted cytokines, the RANTES ELISA kit (#DY278-05; R&D Systems, Minneapolis, MN, United States) and TARC ELISA kit (#DY364-05; R&D Systems, Minneapolis, MN, United States) were used.

    Techniques: Expressing, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control